A 2-aza-Cope reactivity-based platform for ratiometric fluorescence imaging of formaldehyde in living cells.

A 2-aza-Cope reactivity-based platform for ratiometric fluorescence imaging of formaldehyde in living cells† †Electronic supplementary information (ESI) available: Experimental details, including general methods, synthetic details, NMR spectra, in vitro detection limit, and supplemental figures. See DOI: 10.1039/c7sc00748e Click here for additional data file.

Development of a General Aza-Cope Reaction Trigger Applied to Fluorescence Imaging of Formaldehyde in Living Cells

Formaldehyde (FA) is a reactive signaling molecule that is continuously produced through a number of central biological pathways spanning epigenetics to one-carbon metabolism. On the other hand, aberrant, elevated levels of FA are implicated in disease states ranging from asthma to neurodegenerative disorders. In this context, fluorescence-based probes for FA imaging are emerging as potentially...

A coumarin-quinolinium-based fluorescent probe for ratiometric sensing of sulfite in living cells.

Based on a novel coumarin-quinolinium platform, probe 2 was rationally designed and synthesized as a novel ratiometric fluorescent sensor for sulfite anions. The probe exhibited a wide dynamic concentration range for sulfite anions in a PBS buffer (containing 1 mg mL(-1) BSA). More importantly, the probe was suitable for ratiometric fluorescence imaging in living cells with high sensitivity, fa...

Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide.

We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive monitoring of hydrogen sulfide based on a dicyanoisophorone platform. An excellent linear relationship of fluorescence intensity ratio (I637/I558) (R(2) = 0.9867) versus hydrogen sulfide concentration in the range of 1-12 μM was obtained. This probe exhibited a remarkable fluorescence respo...

Cd(II)-terpyridine-based complex as a ratiometric fluorescent probe for pyrophosphate detection in solution and as an imaging agent in living cells.

The terpyridine anthracene ligand was synthesized and characterized. is a ratiometric fluorescent probe for Cd(2+) with a recognition mechanism based on intramolecular charge transfer (ICT). An complex was isolated, and its structure was established using single-crystal XRD. The complex was able to serve as a novel reversible chemosensing ensemble to allow ratiometric response to pyrophosphate ...